Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon
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چکیده
منابع مشابه
Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon
Multi-spanning membrane protein loops are directed alternately into the cytosol or ER lumen during cotranslational integration. Nascent chain exposure is switched after a newly synthesized transmembrane segment (TMS) enters the ribosomal tunnel. FRET measurements revealed that each TMS is initially extended, but folds into a compact conformation after moving 6-7 residues from the peptidyltransf...
متن کاملPolytopic membrane protein folding at L 17 in the ribosome tunnel initiates cyclical
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 195 No. 1 55–70 www.jcb.org/cgi/doi/10.1083/jcb.201103118 JCB 55 Correspondence to Arthur E. Johnson: [email protected] Abbreviations used in this paper: A, acceptor-containing sample; B, sample lacking acceptor and donor; D, donor-containing sample; DA, sample containing donor and acceptor; Ac-Lys, N-acetyl-lysine; ANB-Ly...
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In living systems, polypeptide chains are synthesised on ribosomes, molecular machines composed of over 50 protein and nucleic acid molecules. As nascent chains emerge from the ribosomal exit tunnel and into the cellular environment, the majority must fold into specific structures in order to function. In this article we discuss recent approaches designed to reveal how such folding occurs and r...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2011
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.201103118